ELISA is a really common test that's usually utilized for identifying antibodies, peptides, hormones, and proteins. It's intended for doing immunoassays.
No matter the many benefits, there are several pitfalls that everyone must do before beginning to talk about ELISA or protein assay kit . To know about the best protein assay kit you can search the internet.
Pitfall 1 – Never Use Staying TMB Coloring Development Agent Of the Prior Kit
Stopover here only! It's a really good habit if you maintain the resources safely. But, in regards to using reagents then discontinue their use in various batches together.
That is so because; each reagent that's within the kit is optimized based on the performance. In the event, you use any other reagent of another lot, there's the chance of affecting the result of ELISA.
Additionally, the reagents that got over dated shouldn't be used. One more thing that ought to be kept in mind that it never combines the reagents.
Pitfall 2 -Do Not Take Up The Plates When There Is Not Enough Space In The Incubator
Whilst doing ELISA sample prep, be sure the testing plates should not be stacked upon each other as this activity can hamper the test outcome. When there's insufficient space in the incubator, it contributes to the incidence of edge effect that leads to exposure of spores in various problems.
To prevent such conditions, it's advised to go for replicating or triplicating the assay intended for both standards and samples to create huge discrepancies.
Pitfall 3 -ISome Other Technique To Dry Up The Plate.
If you're considering this, be careful. According to the protocols, the ELISA plates should be tap nicely to remove any kind of residual fluid on absorbent materials.